曾文炉,赵飞飞,曹照根,茹炳根

• 1:北京大学生命科学学院蛋白质工程及植物基因工程国家重点实验室
• 2:南开大学环境科学与工程学院天津市城市生态环境修复与污染防治重点实验室

摘要(Abstract)：

利用海藻酸钠/CaCl2凝胶包埋法固定转小鼠金属硫蛋白-Ⅰ(mMT-Ⅰ)基因聚球藻7002及其野生宿主藻,就固定化藻的生长、对重金属的耐受性及其去除重金属的性能进行了初步研究。结果表明,固定化藻细胞比游离藻细胞生长较慢,培养周期较长,且最大细胞浓度偏低,但固定化可有效提高藻细胞对重金属的耐受性。固定化转mMT-Ⅰ聚球藻对Pb,Cd和Hg的耐受系数(以每单位的细胞吸光值OD750计)分别为6.077,0.610,1.093 mmol/L,而固定化野生聚球藻对于Pb,Cd和Hg的耐受系数分别为1.981,0.170,0.091 mmol/L;固定化转mMT-I聚球藻对重金属的去除效能明显优于游离藻:经3 d的处理,固定化转MT藻对Pb,Cd,Hg的去除率分别为88.09%,81.23%,91.45%,而固定化野生藻在相同的条件下则分别为77.3%,73.47%,85.44%。

关键词(KeyWords)： 小鼠金属硫蛋白-Ⅰ;聚球藻7002;转基因;固定化;重金属

Abstract:

Keywords:

基金项目(Foundation): 国家第33批博士后科学基金项目(2003033079)

作者(Author): 曾文炉,赵飞飞,曹照根,茹炳根

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